Isolation and mapping of a polymorphic DNA sequence (pMCT106) on chromosome 2 [D2S61].

DHPLC Applications: Finding DNA Variation on the Y Chromosome

Denaturing High-Performance Liquid Chromatography (DHPLC) is a recently developed technique forthe detection of single nucleotide polymorphisms (SNPs) and mutations. It involves the comparisonbetween two or more DNAs as a mixture of denatured and reannealed PCR products. The methodologyis based on the principle of reversed phase liquid chromatography and uses a unique DNA sepa...

Isolation and mapping of a polymorphic DNA sequence (pEKMDA2-I) on chromosome 16 [D16S83].

Isolation and mapping of a polymorphic DNA sequence pYNH24 on chromosome 2 (D2S44).

SOURCE/DESCRIPTION; A 2.0 kb Mspl fragment from cosmid YNH24 isolated by HBV-2 oligonucleotide (GGAGTTGGGGGAGGAG) (1) was subcloned into the AccI site of pUC18. POLYMORPHISM: Mspl identifies a >30 allele VNTR polymorphism with bands between 1.0-5.0 kb. Taql, Bglll, Pvull, PstI and BamHI also detect the same polymorphism.

Isolation of polymorphic DNA fragments from human chromosome 4.

We have identified and characterized 40 DNA probes detecting restriction fragment length polymorphism (RFLP) on human chromosome 4. Single copy human clones were isolated from a bacteriophage library enriched for chromosome 4 sequences. Each clone was hybridized to somatic cell hybrid DNAs for verification of its species and chromosomal origin and for regional localization. Sequences specific f...

USE OF VECTORETTE AND SUBVECTORETTE PCR FOR THE ISOLATION OF TERMINAL SEQUENCES FROM Y EAST ARTIFICIAL CHROMOSOME (YAC) CLONES

Development of yeast artificial chromosome (Y AC) vectors, molecular cloning of large segments of chromosomal DNA, and their propagation in yeast cells has become feasible. Overlapping Y AC provides a route to the development of physical maps of entire mammalian chromosomes. A rapid method was developed to isolate and sequence termini of Y AC inserts quickly. The Y AC clone is digested wit...